Functional Assayes

Contacts: Drs. Savita Pahwa (Spahwa@med.miami.edu) and Helena Schmidtmayerova (HSchmidtmayerova@med.miami.edu)    

• Lymphocyte proliferation assay (LPA) in response to mitogens, antigens, and recall antigens detected  by flow cytometry using 4 or 7 color assay
  • CFSE dye dilution
  • Ki7 staining
  • BrdU staining
• IFN-g- producing cells quantified  by ELISPOT
• Microbial translocation assays
  • Plasma LPS
  • Soluble CD14
  • 16S ribosomal DNA
• Intracytoplasmic cytokine detection by flow cytometry

Contact: Dr. Deshratan Asthana (DAsthan@med.miami.edu)

Cytokine & chemokine quantification in cell culture supernatants, plasma, sera and cerebrospinal fluids by

• ELISA
• Multiplex assay by Protein Microarray (detection of 5, 12, or 20 cytokines in 20-50 ml)
• ELISPOT
  

Contact: Dr. Mary Ann Fletcher (MFletche@med.miami.edu)

• NK cells cytotoxicity assay determined by 51Cr release
• Mitogen stimulated cytokine production, plasma and serum cytokines detection using high sensitivity assay
• Quantitative analysis of surface molecules (CD38 and HLADR) and intracellular cytotoxic molecules (granzyme and perforin)